5.14 - Humulin

• A culture of this bacteria will be injected into the fermenter
• It will be necessary to provide this culture:
• Nutrients
• Controlled temperature and pH
• Gases inside the fermenter
• Providing the perfect temperature, pH, gases and the right nutrients will insure optimal growth of the bacteria.
• The population will therefore increase and the bacteria will manufacture the protein Insulin.
• The bacteria inside the fermenter will  manufacture insulin from the nutrient provided (amino acids).
• It will then be necessary to remove the product and carry out purification
• It needs to be purified for human consumption. The ways to purify the insulin is called downstream processing
• The genetically engineered human insulin is called humulin